An experiment measures how temperature affects the rate of an enzyme reaction. The rate doubles when the temperature is increased from 20 ^\circ\text{C} to 30 ^\circ\text{C}. When the temperature is increased further to 60 ^\circ\text{C}, the rate falls to almost zero. Which statement best explains the very low rate at 60 ^\circ\text{C}?

The enzyme is denatured so the active site no longer fits the substrate.

The substrate is used up completely at 60 ^\circ\text{C}.

The enzyme has too little kinetic energy to collide with the substrate.

The pH of the solution must have decreased at 60 ^\circ\text{C}.

BI-1333

Effect of temperature and pH on enzymes

Biology · Secondary 4 · Hard · Effect of temperature and pH on enzymes

An experiment measures how temperature affects the rate of an enzyme reaction. The rate doubles when the temperature is increased from

20 ^\circ\text{C}

30 ^\circ\text{C}

. When the temperature is increased further to

60 ^\circ\text{C}

, the rate falls to almost zero.

Which statement best explains the very low rate at

60 ^\circ\text{C}

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