BI-1361
Effect of temperature and pH on enzymes
Biology · Secondary 4 · Hard · Effect of temperature and pH on enzymes
In an investigation, a student measures how the rate of an enzyme-controlled reaction changes with temperature. She finds that the rate increases from 10 °C to 35 °C, then decreases sharply from 35 °C to 60 °C. Which statement best explains why the rate decreases above 35 °C?
- The enzyme molecules gain more kinetic energy so they collide less often with substrate molecules.
- The enzyme’s active site changes shape so the substrate no longer fits.
- The substrate molecules are used up completely at higher temperatures.
- The enzyme becomes more soluble so it cannot bind to the substrate.
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