BI-1381
Effect of temperature and pH on enzymes
Biology · Secondary 4 · Hard · Effect of temperature and pH on enzymes
In an experiment, a student measures the rate of an enzyme-catalysed reaction at different temperatures. The rate increases from 15 °C to 35 °C, but decreases sharply at 50 °C. Using your knowledge of enzyme activity and temperature, what is the main reason for the decrease at 50 °C?
- The enzyme is denatured so its active site changes shape and cannot bind the substrate effectively.
- The substrate molecules stop moving and can no longer collide with the enzyme.
- The enzyme concentration in the solution decreases as temperature increases.
- The activation energy of the reaction becomes too low for the reaction to proceed.
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