BI-70
Effect of temperature and pH on enzymes
Biology · Secondary 3 · Hard · Effect of temperature and pH on enzymes
The graph shows how the rate of an enzyme‑catalysed reaction changes with temperature. The rate increases from 10 °C to 35 °C, then decreases sharply above 40 °C. Which statement explains the decrease in rate above 40 °C?
- The enzyme is denatured and its active site changes shape.
- The substrate is used up completely at 40 °C.
- The enzyme freezes and cannot move.
- The pH of the solution becomes neutral.
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